A New Tool for In Vivo Study of Astrocyte Connexin 43 in Brain.

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State: Public
Version: author
License: CC BY 4.0
Serval ID
serval:BIB_7C1615ED7809
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A New Tool for In Vivo Study of Astrocyte Connexin 43 in Brain.
Journal
Scientific reports
Author(s)
Droguerre M., Tsurugizawa T., Duchêne A., Portal B., Guiard B.P., Déglon N., Rouach N., Hamon M., Mouthon F., Ciobanu L., Charvériat M.
ISSN
2045-2322 (Electronic)
ISSN-L
2045-2322
Publication state
Published
Issued date
04/12/2019
Peer-reviewed
Oui
Volume
9
Number
1
Pages
18292
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Astrocytes are glial cells organized in dynamic and structured networks in the brain. These plastic networks, involving key proteins such as connexin 43 (Cx43), are engaged in fine neuronal tuning and have recently been considered as emerging therapeutic targets in central nervous system disorders. We developed and validated a new application of the manganese-enhanced magnetic resonance imaging (MEMRI) technique allowing in vivo investigations of astrocyte-neuron interactions through quantification of brain Cx43 functional activity. The proof of concept has been achieved by quantification of MEMRI signals in brain after either local astrocyte-specific Cx43 knockdown with shRNA or systemic administration of Cx43 blockers. Unilateral hippocampal Cx43 genetical silencing was associated with an ipsilateral local increase of MEMRI signal. Furthermore, Cx43 blockers also enhanced MEMRI signal responses in hippocampus. Altogether, these data reveal the MEMRI technique as a tool for quantitative imaging of in vivo Cx43-dependent function in astrocytes under physiological and pathological conditions.
Keywords
Multidisciplinary
Pubmed
Web of science
Open Access
Yes
Create date
11/12/2019 14:09
Last modification date
05/01/2020 7:18
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