Frequency domain lifetime and spectral imaging microscopy.

Détails

ID Serval
serval:BIB_7735C1B13503
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Frequency domain lifetime and spectral imaging microscopy.
Périodique
Microscopy Research and Technique
Auteur(s)
Pelet S., Previte M.J., Kim D., Kim K.H., Su T.T., So P.T.
ISSN
1059-910X (Print)
ISSN-L
1059-910X
Statut éditorial
Publié
Date de publication
2006
Volume
69
Numéro
11
Pages
861-874
Langue
anglais
Résumé
In the femtoliter observation volume of a two-photon microscope, multiple fluorophores can be present and complex photophysics can take place. Combined detection of the fluorescence emission spectra and lifetimes can provide deeper insight into specimen properties than these two imaging modalities taken separately. Therefore, we have developed a detection scheme based on a frequency-modulated multichannel photomultiplier, which measures simultaneously the spectrum and the lifetime of the emitted fluorescence. Experimentally, the efficiency of the frequency domain lifetime measurement was compared to a time domain set-up. The performance of this spectrally and lifetime-resolved microscope was evaluated on reference specimens and living cells labeled with three different stains targeting the membrane, the mitochondria, and the nucleus.
Mots-clé
fluorescence lifetime imaging microscopy, two-photon microscopy, time correlated singe photon counting
Pubmed
Web of science
Création de la notice
25/09/2012 14:41
Dernière modification de la notice
20/08/2019 14:34
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