Frequency domain lifetime and spectral imaging microscopy.

Details

Serval ID
serval:BIB_7735C1B13503
Type
Article: article from journal or magazin.
Collection
Publications
Title
Frequency domain lifetime and spectral imaging microscopy.
Journal
Microscopy Research and Technique
Author(s)
Pelet S., Previte M.J., Kim D., Kim K.H., Su T.T., So P.T.
ISSN
1059-910X (Print)
ISSN-L
1059-910X
Publication state
Published
Issued date
2006
Volume
69
Number
11
Pages
861-874
Language
english
Abstract
In the femtoliter observation volume of a two-photon microscope, multiple fluorophores can be present and complex photophysics can take place. Combined detection of the fluorescence emission spectra and lifetimes can provide deeper insight into specimen properties than these two imaging modalities taken separately. Therefore, we have developed a detection scheme based on a frequency-modulated multichannel photomultiplier, which measures simultaneously the spectrum and the lifetime of the emitted fluorescence. Experimentally, the efficiency of the frequency domain lifetime measurement was compared to a time domain set-up. The performance of this spectrally and lifetime-resolved microscope was evaluated on reference specimens and living cells labeled with three different stains targeting the membrane, the mitochondria, and the nucleus.
Keywords
fluorescence lifetime imaging microscopy, two-photon microscopy, time correlated singe photon counting
Pubmed
Web of science
Create date
25/09/2012 14:41
Last modification date
20/08/2019 14:34
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