Genomic organisation and expression of mouse deoxyribonuclease I

Détails

ID Serval
serval:BIB_75E5C8B6CE2B
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Genomic organisation and expression of mouse deoxyribonuclease I
Périodique
Biochemical and Biophysical Research Communications
Auteur⸱e⸱s
Peitsch  M. C., Irmler  M., French  L. E., Tschopp  J.
ISSN
0006-291X (Print)
Statut éditorial
Publié
Date de publication
02/1995
Volume
207
Numéro
1
Pages
62-8
Notes
Comparative Study
Journal Article --- Old month value: Feb 6
Résumé
Deoxyribonuclease I (DNase I) has recently been implicated in cell death by apoptosis, a process which is frequently accompanied by chromatin DNA degradation. Despite extensive studies on DNase I, its genomic organisation remained unknown. Here we report for the first time on the intron-exon structure of the DNase I gene. The coding region of mouse DNase I is composed of eight introns and eight exons, spanning 2315 base pairs. The deduced protein sequence is 91.5% identical to its rat counterpart, but does not carry the two mutations (Glu13 to D and V67 to I) responsible for the decrease in actin-binding of rat DNase I. The enzymatic activity of mouse DNase I is found in striated muscle, kidney, intestine, liver, lymphnodes, but not in the heart, spleen or pancreas.
Mots-clé
Amino Acid Sequence Animals Base Sequence Cattle DNA Primers DNA, Complementary/chemistry Deoxyribonuclease I/*biosynthesis/chemistry/*genetics Exons *Gene Expression Gene Library Humans Mice/*genetics Molecular Sequence Data Myocardium/enzymology Polymerase Chain Reaction Protein Structure, Secondary Rats Restriction Mapping Sequence Homology, Amino Acid Sheep Swine
Pubmed
Web of science
Création de la notice
24/01/2008 16:19
Dernière modification de la notice
20/08/2019 15:33
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