Genomic organisation and expression of mouse deoxyribonuclease I
Details
Serval ID
serval:BIB_75E5C8B6CE2B
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Genomic organisation and expression of mouse deoxyribonuclease I
Journal
Biochemical and Biophysical Research Communications
ISSN
0006-291X (Print)
Publication state
Published
Issued date
02/1995
Volume
207
Number
1
Pages
62-8
Notes
Comparative Study
Journal Article --- Old month value: Feb 6
Journal Article --- Old month value: Feb 6
Abstract
Deoxyribonuclease I (DNase I) has recently been implicated in cell death by apoptosis, a process which is frequently accompanied by chromatin DNA degradation. Despite extensive studies on DNase I, its genomic organisation remained unknown. Here we report for the first time on the intron-exon structure of the DNase I gene. The coding region of mouse DNase I is composed of eight introns and eight exons, spanning 2315 base pairs. The deduced protein sequence is 91.5% identical to its rat counterpart, but does not carry the two mutations (Glu13 to D and V67 to I) responsible for the decrease in actin-binding of rat DNase I. The enzymatic activity of mouse DNase I is found in striated muscle, kidney, intestine, liver, lymphnodes, but not in the heart, spleen or pancreas.
Keywords
Amino Acid Sequence
Animals
Base Sequence
Cattle
DNA Primers
DNA, Complementary/chemistry
Deoxyribonuclease I/*biosynthesis/chemistry/*genetics
Exons
*Gene Expression
Gene Library
Humans
Mice/*genetics
Molecular Sequence Data
Myocardium/enzymology
Polymerase Chain Reaction
Protein Structure, Secondary
Rats
Restriction Mapping
Sequence Homology, Amino Acid
Sheep
Swine
Pubmed
Web of science
Create date
24/01/2008 15:19
Last modification date
20/08/2019 14:33