A genetically encoded sensor measures temporal oxytocin release from different neuronal compartments.

Détails

ID Serval
serval:BIB_74B667312712
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A genetically encoded sensor measures temporal oxytocin release from different neuronal compartments.
Périodique
Nature biotechnology
Auteur⸱e⸱s
Qian T., Wang H., Wang P., Geng L., Mei L., Osakada T., Wang L., Tang Y., Kania A., Grinevich V., Stoop R., Lin D., Luo M., Li Y.
ISSN
1546-1696 (Electronic)
ISSN-L
1087-0156
Statut éditorial
Publié
Date de publication
07/2023
Peer-reviewed
Oui
Volume
41
Numéro
7
Pages
944-957
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Oxytocin (OT), a peptide hormone and neuromodulator, is involved in diverse physiological and pathophysiological processes in the central nervous system and the periphery. However, the regulation and functional sequences of spatial OT release in the brain remain poorly understood. We describe a genetically encoded G-protein-coupled receptor activation-based (GRAB) OT sensor called GRAB <sub>OT1.0</sub> . In contrast to previous methods, GRAB <sub>OT1.0</sub> enables imaging of OT release ex vivo and in vivo with suitable sensitivity, specificity and spatiotemporal resolution. Using this sensor, we visualize stimulation-induced OT release from specific neuronal compartments in mouse brain slices and discover that N-type calcium channels predominantly mediate axonal OT release, whereas L-type calcium channels mediate somatodendritic OT release. We identify differences in the fusion machinery of OT release for axon terminals versus somata and dendrites. Finally, we measure OT dynamics in various brain regions in mice during male courtship behavior. Thus, GRAB <sub>OT1.0</sub> provides insights into the role of compartmental OT release in physiological and behavioral functions.
Mots-clé
Male, Mice, Animals, Oxytocin/genetics, Neurons, Brain, Signal Transduction, Central Nervous System
Pubmed
Web of science
Création de la notice
10/01/2023 13:17
Dernière modification de la notice
14/12/2023 7:13
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