A genetically encoded sensor measures temporal oxytocin release from different neuronal compartments.
Details
Serval ID
serval:BIB_74B667312712
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A genetically encoded sensor measures temporal oxytocin release from different neuronal compartments.
Journal
Nature biotechnology
ISSN
1546-1696 (Electronic)
ISSN-L
1087-0156
Publication state
Published
Issued date
07/2023
Peer-reviewed
Oui
Volume
41
Number
7
Pages
944-957
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Abstract
Oxytocin (OT), a peptide hormone and neuromodulator, is involved in diverse physiological and pathophysiological processes in the central nervous system and the periphery. However, the regulation and functional sequences of spatial OT release in the brain remain poorly understood. We describe a genetically encoded G-protein-coupled receptor activation-based (GRAB) OT sensor called GRAB <sub>OT1.0</sub> . In contrast to previous methods, GRAB <sub>OT1.0</sub> enables imaging of OT release ex vivo and in vivo with suitable sensitivity, specificity and spatiotemporal resolution. Using this sensor, we visualize stimulation-induced OT release from specific neuronal compartments in mouse brain slices and discover that N-type calcium channels predominantly mediate axonal OT release, whereas L-type calcium channels mediate somatodendritic OT release. We identify differences in the fusion machinery of OT release for axon terminals versus somata and dendrites. Finally, we measure OT dynamics in various brain regions in mice during male courtship behavior. Thus, GRAB <sub>OT1.0</sub> provides insights into the role of compartmental OT release in physiological and behavioral functions.
Keywords
Male, Mice, Animals, Oxytocin/genetics, Neurons, Brain, Signal Transduction, Central Nervous System
Pubmed
Web of science
Create date
10/01/2023 14:17
Last modification date
14/12/2023 8:13