Cellular sensitivity to oxidative stress in the photosensitivity dermatitis/actinic reticuloid syndrome

Détails

ID Serval
serval:BIB_749B3C1B4780
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cellular sensitivity to oxidative stress in the photosensitivity dermatitis/actinic reticuloid syndrome
Périodique
Journal of Investigative Dermatology
Auteur⸱e⸱s
Applegate  L. A., Frenk  E., Gibbs  N., Johnson  B., Ferguson  J., Tyrrell  R. M.
ISSN
0022-202X
Statut éditorial
Publié
Date de publication
05/1994
Peer-reviewed
Oui
Volume
102
Numéro
5
Pages
762-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Résumé
Skin fibroblasts from certain patients with the photosensitivity dermatitis/actinic reticuloid syndrome show enhanced sensitivity to ultraviolet radiation compared to normal fibroblasts. To probe further the link between oxidative damage and this disease, we have obtained a more extensive set of cell lines from patients with a severe form of the disease and examined their sensitivity towards oxidative stress by measuring cell survival following UVA radiation (330-450 nm) or hydrogen peroxide treatment (0.1-2.4 mM). The activation of the stress gene, heme oxygenase, has also been assessed by measuring the accumulation of mRNA after hydrogen peroxide treatment. Our studies have confirmed that a slight ultraviolet sensitivity is a characteristic of photosensitivity dermatitis/actinic reticuloid syndrome cell strains and we further demonstrate that these cell lines are particularly sensitive to hydrogen peroxide with up to a three- to fourfold increased sensitivity as compared to normal controls. We also show that certain ataxia telangiectasia strains that are especially sensitive to hydrogen peroxide are also slightly sensitive to ultraviolet radiation. Hydrogen peroxide induces accumulation of mRNA for the oxidant-inducible stress protein, heme oxygenase, with similar kinetics (maximum mRNA accumulation 2-4 h following treatment) and with a similar range of magnitudes in both normal (6.6-20.6 times mRNA increase over basal levels) and photosensitivity dermatitis/actinic reticuloid (2.9-12.8 times) skin cells. Because cells from photosensitivity dermatitis/actinic reticuloid patients show increased sensitivity towards oxidative stress but show no significant change in oxidant activation of the heme oxygenase gene, we propose that the defect involves a late stage of processing of oxidative damage rather than a compromised free radical scavenging system.
Mots-clé
Adolescent Adult Aged Aged, 80 and over Ataxia Telangiectasia/pathology Cell Survival/drug effects/radiation effects Cells, Cultured Child Child, Preschool Dermatitis, Photoallergic/*pathology Female Fibroblasts/drug effects/*metabolism/radiation effects Gene Expression Regulation/drug effects Heme Oxygenase (Decyclizing)/genetics Humans Hydrogen Peroxide/pharmacology Male Middle Aged Oxidants/pharmacology Photosensitivity Disorders/*pathology RNA, Messenger/analysis Syndrome Ultraviolet Rays
Pubmed
Web of science
Open Access
Oui
Création de la notice
17/01/2008 14:14
Dernière modification de la notice
20/08/2019 14:32
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