Cellular sensitivity to oxidative stress in the photosensitivity dermatitis/actinic reticuloid syndrome
Details
Serval ID
serval:BIB_749B3C1B4780
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cellular sensitivity to oxidative stress in the photosensitivity dermatitis/actinic reticuloid syndrome
Journal
Journal of Investigative Dermatology
ISSN
0022-202X
Publication state
Published
Issued date
05/1994
Peer-reviewed
Oui
Volume
102
Number
5
Pages
762-7
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: May
Research Support, Non-U.S. Gov't --- Old month value: May
Abstract
Skin fibroblasts from certain patients with the photosensitivity dermatitis/actinic reticuloid syndrome show enhanced sensitivity to ultraviolet radiation compared to normal fibroblasts. To probe further the link between oxidative damage and this disease, we have obtained a more extensive set of cell lines from patients with a severe form of the disease and examined their sensitivity towards oxidative stress by measuring cell survival following UVA radiation (330-450 nm) or hydrogen peroxide treatment (0.1-2.4 mM). The activation of the stress gene, heme oxygenase, has also been assessed by measuring the accumulation of mRNA after hydrogen peroxide treatment. Our studies have confirmed that a slight ultraviolet sensitivity is a characteristic of photosensitivity dermatitis/actinic reticuloid syndrome cell strains and we further demonstrate that these cell lines are particularly sensitive to hydrogen peroxide with up to a three- to fourfold increased sensitivity as compared to normal controls. We also show that certain ataxia telangiectasia strains that are especially sensitive to hydrogen peroxide are also slightly sensitive to ultraviolet radiation. Hydrogen peroxide induces accumulation of mRNA for the oxidant-inducible stress protein, heme oxygenase, with similar kinetics (maximum mRNA accumulation 2-4 h following treatment) and with a similar range of magnitudes in both normal (6.6-20.6 times mRNA increase over basal levels) and photosensitivity dermatitis/actinic reticuloid (2.9-12.8 times) skin cells. Because cells from photosensitivity dermatitis/actinic reticuloid patients show increased sensitivity towards oxidative stress but show no significant change in oxidant activation of the heme oxygenase gene, we propose that the defect involves a late stage of processing of oxidative damage rather than a compromised free radical scavenging system.
Keywords
Adolescent
Adult
Aged
Aged, 80 and over
Ataxia Telangiectasia/pathology
Cell Survival/drug effects/radiation effects
Cells, Cultured
Child
Child, Preschool
Dermatitis, Photoallergic/*pathology
Female
Fibroblasts/drug effects/*metabolism/radiation effects
Gene Expression Regulation/drug effects
Heme Oxygenase (Decyclizing)/genetics
Humans
Hydrogen Peroxide/pharmacology
Male
Middle Aged
Oxidants/pharmacology
Photosensitivity Disorders/*pathology
RNA, Messenger/analysis
Syndrome
Ultraviolet Rays
Pubmed
Web of science
Open Access
Yes
Create date
17/01/2008 15:14
Last modification date
20/08/2019 15:32