Development of Sample-Adaptable Holders for Lightsheet Microscopy.

Détails

Ressource 1Télécharger: fnana-13-00026.pdf (1752.86 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY 4.0
ID Serval
serval:BIB_710A13AAFAA0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Development of Sample-Adaptable Holders for Lightsheet Microscopy.
Périodique
Frontiers in Neuroanatomy
Auteur⸱e⸱s
Laroche T., Burri O., Dubey L.K., Seitz A.
ISSN
1662-5129 (Print)
ISSN-L
1662-5129
Statut éditorial
Publié
Date de publication
2019
Peer-reviewed
Oui
Volume
13
Pages
26
Langue
anglais
Résumé
Multi-user core microscopy facilities are often faced with the challenge to adapt or modify existing instruments. This is essential in order to fulfill the requirements of the user community, who wants to image a wide range of model organisms with varying stains and sample thicknesses. In recent years, lightsheet microscopy has turned into an invaluable tool for both live and cleared sample imaging of many different specimens. This brought up new challenges in terms of sample mounting as the classical approach of attachment onto a coverslip cannot be universally applied. Here we describe the development of a diversified holder which extends the range of samples which can be imaged on a Zeiss Lightsheet microscope Z1. We focus on mounting strategies of cleared specimens; however, the holder and mounting strategy can be applied to live specimens too. The proposed methodology provides very high flexibility along with numerous possibilities for adaptation based on imaging specimen size, condition and available clearing reagents. Moreover, the described mounting strategies can be applied to other light sheet microscopes that can mount 1 mL syringes.
Mots-clé
clearing, custom, fluorescence, imaging, lightsheet microscopy, lymph node, microscopy, sample preparation
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/04/2019 7:15
Dernière modification de la notice
20/08/2019 14:29
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