Development of Sample-Adaptable Holders for Lightsheet Microscopy.

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Version: Final published version
License: CC BY 4.0
Serval ID
serval:BIB_710A13AAFAA0
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Development of Sample-Adaptable Holders for Lightsheet Microscopy.
Journal
Frontiers in Neuroanatomy
Author(s)
Laroche T., Burri O., Dubey L.K., Seitz A.
ISSN
1662-5129 (Print)
ISSN-L
1662-5129
Publication state
Published
Issued date
2019
Peer-reviewed
Oui
Volume
13
Pages
26
Language
english
Abstract
Multi-user core microscopy facilities are often faced with the challenge to adapt or modify existing instruments. This is essential in order to fulfill the requirements of the user community, who wants to image a wide range of model organisms with varying stains and sample thicknesses. In recent years, lightsheet microscopy has turned into an invaluable tool for both live and cleared sample imaging of many different specimens. This brought up new challenges in terms of sample mounting as the classical approach of attachment onto a coverslip cannot be universally applied. Here we describe the development of a diversified holder which extends the range of samples which can be imaged on a Zeiss Lightsheet microscope Z1. We focus on mounting strategies of cleared specimens; however, the holder and mounting strategy can be applied to live specimens too. The proposed methodology provides very high flexibility along with numerous possibilities for adaptation based on imaging specimen size, condition and available clearing reagents. Moreover, the described mounting strategies can be applied to other light sheet microscopes that can mount 1 mL syringes.
Keywords
clearing, custom, fluorescence, imaging, lightsheet microscopy, lymph node, microscopy, sample preparation
Pubmed
Web of science
Open Access
Yes
Create date
15/04/2019 7:15
Last modification date
20/08/2019 14:29
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