Branch migration and Holliday junction resolution catalyzed by activities from mammalian cells

Détails

ID Serval
serval:BIB_6E291577BB5A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Branch migration and Holliday junction resolution catalyzed by activities from mammalian cells
Périodique
Cell
Auteur(s)
Constantinou  A., Davies  A. A., West  S. C.
ISSN
0092-8674 (Print)
Statut éditorial
Publié
Date de publication
01/2001
Volume
104
Numéro
2
Pages
259-68
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 26
Résumé
During homologous recombination, DNA strand exchange leads to Holliday junction formation. The movement, or branch migration, of this junction along DNA extends the length of the heteroduplex joint. In prokaryotes, branch migration and Holliday junction resolution are catalyzed by the RuvA and RuvB proteins, which form a complex with RuvC resolvase to form a "resolvasome". Mammalian cell-free extracts have now been fractionated to reveal analogous activities. An ATP-dependent branch migration activity, which migrates junctions through >2700 bp, cofractionates with the Holliday junction resolvase during several chromatographic steps. Together, the two activities promote concerted branch migration/resolution reactions similar to those catalyzed by E. coli RuvABC, highlighting the preservation of this essential pathway in recombination and DNA repair from prokaryotes to mammals.
Mots-clé
Animals Bacterial Proteins/metabolism Cell Fractionation Cell Line Cell-Free System Cricetinae DNA/*metabolism *DNA Helicases DNA Repair DNA-Binding Proteins/genetics/*metabolism Endodeoxyribonucleases/genetics/*metabolism Escherichia coli/chemistry *Escherichia coli Proteins Holliday Junction Resolvases Humans Macromolecular Substances Nucleic Acid Conformation Rabbits *Recombination, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 14:50
Dernière modification de la notice
20/08/2019 14:27
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