Branch migration and Holliday junction resolution catalyzed by activities from mammalian cells
Details
Serval ID
serval:BIB_6E291577BB5A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Branch migration and Holliday junction resolution catalyzed by activities from mammalian cells
Journal
Cell
ISSN
0092-8674 (Print)
Publication state
Published
Issued date
01/2001
Volume
104
Number
2
Pages
259-68
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Jan 26
Research Support, Non-U.S. Gov't --- Old month value: Jan 26
Abstract
During homologous recombination, DNA strand exchange leads to Holliday junction formation. The movement, or branch migration, of this junction along DNA extends the length of the heteroduplex joint. In prokaryotes, branch migration and Holliday junction resolution are catalyzed by the RuvA and RuvB proteins, which form a complex with RuvC resolvase to form a "resolvasome". Mammalian cell-free extracts have now been fractionated to reveal analogous activities. An ATP-dependent branch migration activity, which migrates junctions through >2700 bp, cofractionates with the Holliday junction resolvase during several chromatographic steps. Together, the two activities promote concerted branch migration/resolution reactions similar to those catalyzed by E. coli RuvABC, highlighting the preservation of this essential pathway in recombination and DNA repair from prokaryotes to mammals.
Keywords
Animals
Bacterial Proteins/metabolism
Cell Fractionation
Cell Line
Cell-Free System
Cricetinae
DNA/*metabolism
*DNA Helicases
DNA Repair
DNA-Binding Proteins/genetics/*metabolism
Endodeoxyribonucleases/genetics/*metabolism
Escherichia coli/chemistry
*Escherichia coli Proteins
Holliday Junction Resolvases
Humans
Macromolecular Substances
Nucleic Acid Conformation
Rabbits
*Recombination, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 14:50
Last modification date
20/08/2019 14:27