Multiple regions of TBP participate in the response to transcriptional activators in vivo.

Détails

ID Serval
serval:BIB_6C63DC4D3FD4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Multiple regions of TBP participate in the response to transcriptional activators in vivo.
Périodique
Genes and Development
Auteur⸱e⸱s
Tansey W.P., Ruppert S., Tjian R., Herr W.
ISSN
0890-9369[print], 0890-9369[linking]
Statut éditorial
Publié
Date de publication
11/1994
Volume
8
Numéro
22
Pages
2756-2769
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
We used mutant yeast and human TBP molecules with an altered DNA-binding specificity to examine the role of TBP in transcriptional activation in vivo. We show that yeast TBP is functionally equivalent to human TBP for response to numerous transcriptional activators in human cells, including those that do not function in yeast. Despite the extensive conservation of TBP, its ability to respond to transcriptional activators in vivo is curiously resistant to clustered sets of alanine substitution mutations in different regions of the protein, including those that disrupt DNA binding and basal transcription in vitro. Combined sets of these mutations, however, can attenuate the in vivo activity of TBP and can differentially affect response to different activation domains. Although the activity of TBP mutants in vivo did not correlate with DNA binding or basal transcription in vitro, it did correlate with binding in vitro to the largest subunit of TFIID, hTAFII250. Together, these data suggest that TBP utilizes multiple interactions across its surface to respond to RNA polymerase II transcriptional activators in vivo; some of these interactions appear to involve recruitment of TBP into TFIID, whereas others are involved in response to specific types of transcriptional activators.
Mots-clé
Gene Expression Regulation, Humans, Models, Molecular, Mutation, RNA Polymerase II/biosynthesis, TATA Box, Transcription Factor TFIID, Transcription Factors/genetics, Transcription, Genetic
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 16:36
Dernière modification de la notice
20/08/2019 15:26
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