Multiple regions of TBP participate in the response to transcriptional activators in vivo.

Details

Serval ID
serval:BIB_6C63DC4D3FD4
Type
Article: article from journal or magazin.
Collection
Publications
Title
Multiple regions of TBP participate in the response to transcriptional activators in vivo.
Journal
Genes and Development
Author(s)
Tansey W.P., Ruppert S., Tjian R., Herr W.
ISSN
0890-9369[print], 0890-9369[linking]
Publication state
Published
Issued date
11/1994
Volume
8
Number
22
Pages
2756-2769
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
We used mutant yeast and human TBP molecules with an altered DNA-binding specificity to examine the role of TBP in transcriptional activation in vivo. We show that yeast TBP is functionally equivalent to human TBP for response to numerous transcriptional activators in human cells, including those that do not function in yeast. Despite the extensive conservation of TBP, its ability to respond to transcriptional activators in vivo is curiously resistant to clustered sets of alanine substitution mutations in different regions of the protein, including those that disrupt DNA binding and basal transcription in vitro. Combined sets of these mutations, however, can attenuate the in vivo activity of TBP and can differentially affect response to different activation domains. Although the activity of TBP mutants in vivo did not correlate with DNA binding or basal transcription in vitro, it did correlate with binding in vitro to the largest subunit of TFIID, hTAFII250. Together, these data suggest that TBP utilizes multiple interactions across its surface to respond to RNA polymerase II transcriptional activators in vivo; some of these interactions appear to involve recruitment of TBP into TFIID, whereas others are involved in response to specific types of transcriptional activators.
Keywords
Gene Expression Regulation, Humans, Models, Molecular, Mutation, RNA Polymerase II/biosynthesis, TATA Box, Transcription Factor TFIID, Transcription Factors/genetics, Transcription, Genetic
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 16:36
Last modification date
20/08/2019 15:26
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