Method for confirmation of synthetic corticosteroids in doping urine samples by liquid chromatography-electrospray ionisation mass spectrometry

Détails

ID Serval
serval:BIB_69D2FE367057
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Method for confirmation of synthetic corticosteroids in doping urine samples by liquid chromatography-electrospray ionisation mass spectrometry
Périodique
Journal of Chromatography A
Auteur⸱e⸱s
Fluri  K., Rivier  L., Dienes-Nagy  A., You  C. H., Maitre  A., Schweizer  C., Saugy  M., Mangin  P.
ISSN
0021-9673
Statut éditorial
Publié
Date de publication
2001
Peer-reviewed
Oui
Volume
926
Numéro
1
Pages
87-95
Résumé
In this study, we report on the development of a method to confirm simultaneously nine of the most commonly abused synthetic corticosteroids in urine based on liquid chromatography-electrospray ionisation mass spectrometry. A considerable simplified sample preparation procedure, including liquid-liquid phase extraction with Extrelut-NT3 columns, provided both excellent sample purification and high overall recoveries. Complete HPLC separations were obtained on a reversed-phase column with 1 mM ammonium acetate-acetonitrile (60:40, v/v) as mobile phase. Mass spectral acquisition was done in the negative ion, and selected ion monitoring modes to identify the drugs with at least three characteristic ions. Detection limits were determined at less than or equal to1 ng/ml and the confirmation limits at 1 to 5 ng/ml.
Pubmed
Web of science
Création de la notice
08/02/2008 17:44
Dernière modification de la notice
20/08/2019 14:24
Données d'usage