Cryopreservation of human brain tissue allowing timely production of viable adult human brain cells for autologous transplantation
Détails
ID Serval
serval:BIB_66B0B7CC9C2D
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Cryopreservation of human brain tissue allowing timely production of viable adult human brain cells for autologous transplantation
Périodique
Cryobiology
ISSN
0011-2240
Statut éditorial
Publié
Date de publication
10/2003
Peer-reviewed
Oui
Volume
47
Numéro
2
Pages
179-83
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Research Support, Non-U.S. Gov't --- Old month value: Oct
Résumé
BACKGROUND: Autologous transplantation is an attractive approach to treat some neurological diseases. A major obstacle is the capacity to produce cells for transplantation at the appropriate time. We describe a cryopreservation procedure for adult human brain tissue allowing the generation of cells in vitro. METHODS: Neurological resections were dissected to separate white and grey matter. Fractions were frozen in a specific cryopreservation medium containing a selected serum and stored in liquid nitrogen. Tissue was thawed, cells were mechanically dissociated, expanded in culture and characterized by immunochemistry. RESULTS: Adult human brain tissue cryopreserved for up to two years was successfully used to generate brain cells that could be maintained in culture for up to 100 days. Cells expressed a variety of neuroectodermal markers including GFAP, S100beta, and neurofilament. CONCLUSION: A successful procedure for cryopreservation of adult human brain tissue has been established that might facilitate future autologous transplantation strategies.
Mots-clé
Adult
Brain/*cytology/*pathology
Cell Culture Techniques/*methods
Cell Transplantation
Cryopreservation/*methods
Glial Fibrillary Acidic Protein/biosynthesis
Humans
Immunohistochemistry
Microscopy, Fluorescence
Neurofilament Proteins/metabolism
Nitrogen
Organ Preservation/*methods
S100 Proteins/biosynthesis
Temperature
Time Factors
Transplantation, Autologous
Pubmed
Web of science
Création de la notice
06/02/2008 10:44
Dernière modification de la notice
20/08/2019 15:22