Cryopreservation of human brain tissue allowing timely production of viable adult human brain cells for autologous transplantation

Details

Serval ID
serval:BIB_66B0B7CC9C2D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cryopreservation of human brain tissue allowing timely production of viable adult human brain cells for autologous transplantation
Journal
Cryobiology
Author(s)
Brunet  J. F., Pellerin  L., Magistretti  P., Villemure  J. G.
ISSN
0011-2240
Publication state
Published
Issued date
10/2003
Peer-reviewed
Oui
Volume
47
Number
2
Pages
179-83
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Abstract
BACKGROUND: Autologous transplantation is an attractive approach to treat some neurological diseases. A major obstacle is the capacity to produce cells for transplantation at the appropriate time. We describe a cryopreservation procedure for adult human brain tissue allowing the generation of cells in vitro. METHODS: Neurological resections were dissected to separate white and grey matter. Fractions were frozen in a specific cryopreservation medium containing a selected serum and stored in liquid nitrogen. Tissue was thawed, cells were mechanically dissociated, expanded in culture and characterized by immunochemistry. RESULTS: Adult human brain tissue cryopreserved for up to two years was successfully used to generate brain cells that could be maintained in culture for up to 100 days. Cells expressed a variety of neuroectodermal markers including GFAP, S100beta, and neurofilament. CONCLUSION: A successful procedure for cryopreservation of adult human brain tissue has been established that might facilitate future autologous transplantation strategies.
Keywords
Adult Brain/*cytology/*pathology Cell Culture Techniques/*methods Cell Transplantation Cryopreservation/*methods Glial Fibrillary Acidic Protein/biosynthesis Humans Immunohistochemistry Microscopy, Fluorescence Neurofilament Proteins/metabolism Nitrogen Organ Preservation/*methods S100 Proteins/biosynthesis Temperature Time Factors Transplantation, Autologous
Pubmed
Web of science
Create date
06/02/2008 9:44
Last modification date
20/08/2019 14:22
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