Cryopreservation of human brain tissue allowing timely production of viable adult human brain cells for autologous transplantation
Details
Serval ID
serval:BIB_66B0B7CC9C2D
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Cryopreservation of human brain tissue allowing timely production of viable adult human brain cells for autologous transplantation
Journal
Cryobiology
ISSN
0011-2240
Publication state
Published
Issued date
10/2003
Peer-reviewed
Oui
Volume
47
Number
2
Pages
179-83
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Oct
Research Support, Non-U.S. Gov't --- Old month value: Oct
Abstract
BACKGROUND: Autologous transplantation is an attractive approach to treat some neurological diseases. A major obstacle is the capacity to produce cells for transplantation at the appropriate time. We describe a cryopreservation procedure for adult human brain tissue allowing the generation of cells in vitro. METHODS: Neurological resections were dissected to separate white and grey matter. Fractions were frozen in a specific cryopreservation medium containing a selected serum and stored in liquid nitrogen. Tissue was thawed, cells were mechanically dissociated, expanded in culture and characterized by immunochemistry. RESULTS: Adult human brain tissue cryopreserved for up to two years was successfully used to generate brain cells that could be maintained in culture for up to 100 days. Cells expressed a variety of neuroectodermal markers including GFAP, S100beta, and neurofilament. CONCLUSION: A successful procedure for cryopreservation of adult human brain tissue has been established that might facilitate future autologous transplantation strategies.
Keywords
Adult
Brain/*cytology/*pathology
Cell Culture Techniques/*methods
Cell Transplantation
Cryopreservation/*methods
Glial Fibrillary Acidic Protein/biosynthesis
Humans
Immunohistochemistry
Microscopy, Fluorescence
Neurofilament Proteins/metabolism
Nitrogen
Organ Preservation/*methods
S100 Proteins/biosynthesis
Temperature
Time Factors
Transplantation, Autologous
Pubmed
Web of science
Create date
06/02/2008 10:44
Last modification date
20/08/2019 15:22