Biochemical, morphological and content characterization of synaptobrevin 2-expressing vesicles in astrocytes

Détails

ID Serval
serval:BIB_6023E8A30531
Type
Actes de conférence (partie): contribution originale à la littérature scientifique, publiée à l'occasion de conférences scientifiques, dans un ouvrage de compte-rendu (proceedings), ou dans l'édition spéciale d'un journal reconnu (conference proceedings).
Sous-type
Abstract (résumé de présentation): article court qui reprend les éléments essentiels présentés à l'occasion d'une conférence scientifique dans un poster ou lors d'une intervention orale.
Collection
Publications
Institution
Titre
Biochemical, morphological and content characterization of synaptobrevin 2-expressing vesicles in astrocytes
Titre de la conférence
21st Biennial Meeting of the International Society for Neurochemistry and the 38th Annual Meeting of the American Society for Neurochemistry
Auteur(s)
Martineau M., Puyal J., Koch P., Meulemans A., Hopkins S., Theodosis D.T., Jahn R., Mothet J.P.
Organisation
Cancún, Mexico, August 19-24, 2007
ISBN
0022-3042
Statut éditorial
Publié
Date de publication
2007
Peer-reviewed
Oui
Volume
102
Série
Journal of Neurochemistry
Pages
284
Langue
anglais
Notes
Document Type: Meeting Abstract
Résumé
Neuron-astrocyte reciprocal communication at synapses has emerged as a novel signalling pathway in brain function. Astrocytes sense the level of synaptic activity and, in turn, influence its efficacy through the regulated release of ''glio- transmitters'' such as glutamate, ATP or D-serine. A calcium- dependent exocytosis is proposed to drive the release of gliotransmitters but its existence is still debated. To shed light onto the mechanisms controlling the storage and the release of gliotransmitters and namely D-serine, we have developed a new method for the immunoisolation of synaptobrevin 2-positive vesicles from rat cortical astrocytes in culture. The purified organelles are clear round shape vesicles of excellent purity as judged by electron microscopy. Immunoblotting analysis revealed that isolated vesicles contain most of the major proteins already described for neuron-derived vesicles. In addition, we have analyzed the content for various amino acids of these vesicles by means of chiral capillary electro- phoresis coupled to laser-induced fluorescence detection and liquid chromatography coupled to mass spectrometry. Post- embedding immunogold labelling of the rat neocortex and hippocampus further revealed the expression of D-serine and glutamate in astrocyte processes contacting excitatory sy- napses. Our results provide significant support for the existence of secretory glial vesicles storing chemical substances like D- serine and glutamate and thus point to the co-release of amino acids by exocytosis in astrocytes.
Web of science
Open Access
Oui
Création de la notice
08/02/2010 15:43
Dernière modification de la notice
20/08/2019 15:17
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