Increasing the carbon flux toward synthesis of short-chain-length--medium-chain-length polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae through modification of the beta-oxidation cycle.
Détails
ID Serval
serval:BIB_5EF61209EA9A
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Increasing the carbon flux toward synthesis of short-chain-length--medium-chain-length polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae through modification of the beta-oxidation cycle.
Périodique
Applied and Environmental Microbiology
ISSN
0099-2240 (Print)
ISSN-L
0099-2240
Statut éditorial
Publié
Date de publication
2004
Volume
70
Numéro
9
Pages
5685-5687
Langue
anglais
Résumé
Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by using a mutant of the beta-oxidation-associated multifunctional enzyme with low dehydrogenase activity toward R-3-hydroxybutyryl coenzyme A.
Mots-clé
Acyltransferases/genetics, Acyltransferases/metabolism, Aeromonas/enzymology, Aeromonas/genetics, Carbon/metabolism, Cloning, Molecular, Cupriavidus necator/enzymology, Cupriavidus necator/genetics, DNA, Fungal/genetics, DNA, Fungal/metabolism, Oxidation-Reduction, Peroxisomes/metabolism, Recombinant Proteins/metabolism, Saccharomyces cerevisiae/metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 19:43
Dernière modification de la notice
20/08/2019 14:16
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