Increasing the carbon flux toward synthesis of short-chain-length--medium-chain-length polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae through modification of the beta-oxidation cycle.
Details
Serval ID
serval:BIB_5EF61209EA9A
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Increasing the carbon flux toward synthesis of short-chain-length--medium-chain-length polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae through modification of the beta-oxidation cycle.
Journal
Applied and Environmental Microbiology
ISSN
0099-2240 (Print)
ISSN-L
0099-2240
Publication state
Published
Issued date
2004
Volume
70
Number
9
Pages
5685-5687
Language
english
Abstract
Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by using a mutant of the beta-oxidation-associated multifunctional enzyme with low dehydrogenase activity toward R-3-hydroxybutyryl coenzyme A.
Keywords
Acyltransferases/genetics, Acyltransferases/metabolism, Aeromonas/enzymology, Aeromonas/genetics, Carbon/metabolism, Cloning, Molecular, Cupriavidus necator/enzymology, Cupriavidus necator/genetics, DNA, Fungal/genetics, DNA, Fungal/metabolism, Oxidation-Reduction, Peroxisomes/metabolism, Recombinant Proteins/metabolism, Saccharomyces cerevisiae/metabolism
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 19:43
Last modification date
20/08/2019 14:16
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