Role of enzyme-treated cells in RBC antibody screening using the gel test: a study of anti-RH1, -RH2, and -RH3 antibodies

Détails

ID Serval
serval:BIB_5D42AE3BD34E
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Role of enzyme-treated cells in RBC antibody screening using the gel test: a study of anti-RH1, -RH2, and -RH3 antibodies
Périodique
Journal of Clinical Laboratory Analysis
Auteur⸱e⸱s
Conne  J., Schneider  P., Tissot  J. D.
ISSN
0887-8013 (Print)
Statut éditorial
Publié
Date de publication
2007
Volume
21
Numéro
2
Pages
61-6
Notes
Journal Article
Résumé
The role of enzyme-treated cells (ETCs) in red blood cell (RBC) antibody screening has been the subject of controversy, and its place in the clinical routine remains to be determined. In this work, plasma samples containing anti-RH1 (anti-D; N = 10), anti-RH2 (anti-C; N = 10), or anti-RH3 (anti-E; N = 10) antibodies were studied. The samples were diluted in nonbuffered or buffered normal saline, as well as in a pool of AB plasma samples. Titers and scores were determined by means of the gel test, using the indirect antiglobulin test (IAT) as well as ETCs, with R(0)r, r'r, or r''r test cells. Our results showed that compared to the IAT, ETCs allowed a clearer detection of anti-RH2 and anti-RH3, but not of anti-RH1 antibodies. Based on our study, it is not clear whether the ETC phase of the gel test should be maintained for RBC antibody screening.
Mots-clé
Autoantibodies/*blood Blood Grouping and Crossmatching/*methods Coombs' Test Erythrocytes/drug effects/*immunology Gels Humans Immunoassay/methods Papain/pharmacology Rh-Hr Blood-Group System/*immunology
Pubmed
Web of science
Création de la notice
25/01/2008 16:34
Dernière modification de la notice
20/08/2019 15:15
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