Role of enzyme-treated cells in RBC antibody screening using the gel test: a study of anti-RH1, -RH2, and -RH3 antibodies

Details

Serval ID
serval:BIB_5D42AE3BD34E
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Role of enzyme-treated cells in RBC antibody screening using the gel test: a study of anti-RH1, -RH2, and -RH3 antibodies
Journal
Journal of Clinical Laboratory Analysis
Author(s)
Conne  J., Schneider  P., Tissot  J. D.
ISSN
0887-8013 (Print)
Publication state
Published
Issued date
2007
Volume
21
Number
2
Pages
61-6
Notes
Journal Article
Abstract
The role of enzyme-treated cells (ETCs) in red blood cell (RBC) antibody screening has been the subject of controversy, and its place in the clinical routine remains to be determined. In this work, plasma samples containing anti-RH1 (anti-D; N = 10), anti-RH2 (anti-C; N = 10), or anti-RH3 (anti-E; N = 10) antibodies were studied. The samples were diluted in nonbuffered or buffered normal saline, as well as in a pool of AB plasma samples. Titers and scores were determined by means of the gel test, using the indirect antiglobulin test (IAT) as well as ETCs, with R(0)r, r'r, or r''r test cells. Our results showed that compared to the IAT, ETCs allowed a clearer detection of anti-RH2 and anti-RH3, but not of anti-RH1 antibodies. Based on our study, it is not clear whether the ETC phase of the gel test should be maintained for RBC antibody screening.
Keywords
Autoantibodies/*blood Blood Grouping and Crossmatching/*methods Coombs' Test Erythrocytes/drug effects/*immunology Gels Humans Immunoassay/methods Papain/pharmacology Rh-Hr Blood-Group System/*immunology
Pubmed
Web of science
Create date
25/01/2008 15:34
Last modification date
20/08/2019 14:15
Usage data