The Contribution of lincRNAs at the Interface between Cell Cycle Regulation and Cell State Maintenance.
Détails
Télécharger: 32619701_BIB_5B6E0016A467.pdf (1445.63 [Ko])
Etat: Public
Version: Final published version
Licence: CC BY-NC-ND 4.0
Etat: Public
Version: Final published version
Licence: CC BY-NC-ND 4.0
ID Serval
serval:BIB_5B6E0016A467
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
The Contribution of lincRNAs at the Interface between Cell Cycle Regulation and Cell State Maintenance.
Périodique
iScience
ISSN
2589-0042 (Electronic)
ISSN-L
2589-0042
Statut éditorial
Publié
Date de publication
24/07/2020
Peer-reviewed
Oui
Volume
23
Numéro
7
Pages
101291
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Publication Status: ppublish
Résumé
Cell cycle progression is controlled by the interplay of established cell cycle regulators. Changes in these regulators' activity underpin differences in cell cycle kinetics between cell types. We investigated whether long intergenic noncoding RNAs (lincRNAs) contribute to embryonic stem cell cycle adaptations. Using single-cell RNA sequencing data for mouse embryonic stem cells (mESCs) staged as G1, S, or G2/M we found differentially expressed lincRNAs are enriched among cell cycle-regulated genes. These lincRNAs (CC-lincRNAs) are co-expressed with genes involved in cell cycle regulation. We tested the impact of two CC-lincRNA candidates and show using CRISPR activation that increasing their expression is associated with deregulated cell cycle progression. Interestingly, CC-lincRNAs are often differentially expressed between G1 and S, their promoters are enriched in pluripotency transcription factor (TF) binding sites, and their transcripts are frequently co-regulated with genes involved in the maintenance of pluripotency, suggesting a contribution of CC-lincRNAs to mESC cell cycle adaptations.
Mots-clé
Cell Biology, Molecular Biology
Pubmed
Web of science
Open Access
Oui
Création de la notice
08/07/2020 11:54
Dernière modification de la notice
09/08/2024 14:59