The Contribution of lincRNAs at the Interface between Cell Cycle Regulation and Cell State Maintenance.

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Version: Final published version
License: CC BY-NC-ND 4.0
Serval ID
serval:BIB_5B6E0016A467
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
The Contribution of lincRNAs at the Interface between Cell Cycle Regulation and Cell State Maintenance.
Journal
iScience
Author(s)
Biasini A., Smith AAT (co-first), Abdulkarim B., Ferreira da Silva M., Tan J.Y., Marques A.C.
ISSN
2589-0042 (Electronic)
ISSN-L
2589-0042
Publication state
Published
Issued date
24/07/2020
Peer-reviewed
Oui
Volume
23
Number
7
Pages
101291
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Cell cycle progression is controlled by the interplay of established cell cycle regulators. Changes in these regulators' activity underpin differences in cell cycle kinetics between cell types. We investigated whether long intergenic noncoding RNAs (lincRNAs) contribute to embryonic stem cell cycle adaptations. Using single-cell RNA sequencing data for mouse embryonic stem cells (mESCs) staged as G1, S, or G2/M we found differentially expressed lincRNAs are enriched among cell cycle-regulated genes. These lincRNAs (CC-lincRNAs) are co-expressed with genes involved in cell cycle regulation. We tested the impact of two CC-lincRNA candidates and show using CRISPR activation that increasing their expression is associated with deregulated cell cycle progression. Interestingly, CC-lincRNAs are often differentially expressed between G1 and S, their promoters are enriched in pluripotency transcription factor (TF) binding sites, and their transcripts are frequently co-regulated with genes involved in the maintenance of pluripotency, suggesting a contribution of CC-lincRNAs to mESC cell cycle adaptations.
Keywords
Cell Biology, Molecular Biology
Pubmed
Web of science
Open Access
Yes
Create date
08/07/2020 11:54
Last modification date
09/08/2024 14:59
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