mTORC2 regulates PGE2-mediated endothelial cell survival and migration.

Détails

ID Serval
serval:BIB_560ED2F49720
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
mTORC2 regulates PGE2-mediated endothelial cell survival and migration.
Périodique
Biochemical and Biophysical Research Communications
Auteur(s)
Dada S., Demartines N., Dormond O.
ISSN
1090-2104[electronic], 0006-291X[linking]
Statut éditorial
Publié
Date de publication
2008
Volume
372
Numéro
4
Pages
875-879
Langue
anglais
Notes
Publication types: Journal ArticlePublication Status: ppublish
Résumé
Prostaglandin E(2) (PGE(2)) promotes angiogenesis by in part inducing endothelial cell survival and migration. The present study examined the role of mTOR and its two complexes, mTORC1 and mTORC2, in PGE(2)-mediated endothelial cell responses. We used small interfering RNA (siRNA) to raptor or rictor to block mTORC1 or mTORC2, respectively. We observed that down-regulation of mTORC2 but not mTORC1 reduced baseline and PGE(2)-induced endothelial cell survival and migration. At the molecular level, we found that knockdown of mTORC2 inhibited PGE(2)-mediated Rac and Akt activation two important signaling intermediaries in endothelial cell migration and survival, respectively. In addition, inhibition of mTORC2 by prolonged exposure of endothelial cells to rapamycin also prevented PGE(2)-mediated endothelial cell survival and migration confirming the results obtained with the siRNA approach. Taken together these results show that mTORC2 but not mTORC1 is an important signaling intermediary in PGE(2)-mediated endothelial cell responses.
Mots-clé
Cell Movement, Cell Survival, Dinoprostone/metabolism, Dinoprostone/pharmacology, Endothelial Cells/drug effects, Endothelial Cells/metabolism, Humans, Multienzyme Complexes/antagonists & inhibitors, Multienzyme Complexes/genetics, Neovascularization, Physiologic/genetics, Proto-Oncogene Proteins c-akt/metabolism, RNA, Small Interfering/genetics, Transcription Factors/antagonists & inhibitors, Transcription Factors/genetics, rac GTP-Binding Proteins/metabolism
Pubmed
Web of science
Création de la notice
17/02/2010 11:18
Dernière modification de la notice
20/08/2019 15:10
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