A non-invasive assessment of hepatic glycogen kinetics and post-absorptive gluconeogenesis in man.

Détails

ID Serval
serval:BIB_54A2C176A399
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A non-invasive assessment of hepatic glycogen kinetics and post-absorptive gluconeogenesis in man.
Périodique
Diabetologia
Auteur(s)
Gay L.J., Schneiter P., Schutz Y., Di Vetta V., Jéquier E., Tappy L.
ISSN
0012-186X (Print)
ISSN-L
0012-186X
Statut éditorial
Publié
Date de publication
1994
Volume
37
Numéro
5
Pages
517-523
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
A novel approach to the study of hepatic glycogen kinetics and fractional gluconeogenesis in vivo is described. Ten healthy female subjects were fed an iso-caloric diet containing 55% carbohydrate energy with a 13C abundance of 1.083 atom percent for a 3-day baseline period; then, a diet of similar composition, but providing carbohydrate with a 13C abundance of 1.093 atom percent was started and continued for 5 days. Resting respiratory gas exchanges, urinary nitrogen excretion, breath 13CO2 and plasma 13C glucose were measured every morning in the fasting state. The enrichment in 13C of hepatic glycogen was calculated from these measured data. 13C glycogen enrichment increased after switching to a 13C enriched carbohydrate diet, and was identical to the 13C enrichment of dietary carbohydrates after 3 days. The time required to renew 50% of hepatic glycogen, as determined from the kinetics of 13C glycogen enrichment, was 18.9 +/- 3.6 h. Fractional gluconeogenesis, as determined from the difference between the enrichments of glucose oxidized originating from hepatic glycogen and plasma glucose 13C was 50.8 +/- 5.3%. This non-invasive method will allow the study of hepatic glycogen metabolism in insulin-resistant patients.
Mots-clé
Adult, Carbon Dioxide/analysis, Carbon Isotopes, Dietary Carbohydrates, Female, Gluconeogenesis, Humans, Insulin Resistance, Isotope Labeling/methods, Kinetics, Liver/metabolism, Liver Glycogen/metabolism, Reference Values
Pubmed
Web of science
Open Access
Oui
Création de la notice
24/01/2008 13:36
Dernière modification de la notice
20/08/2019 14:09
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