A non-invasive assessment of hepatic glycogen kinetics and post-absorptive gluconeogenesis in man.

Details

Serval ID
serval:BIB_54A2C176A399
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A non-invasive assessment of hepatic glycogen kinetics and post-absorptive gluconeogenesis in man.
Journal
Diabetologia
Author(s)
Gay L.J., Schneiter P., Schutz Y., Di Vetta V., Jéquier E., Tappy L.
ISSN
0012-186X (Print)
ISSN-L
0012-186X
Publication state
Published
Issued date
1994
Volume
37
Number
5
Pages
517-523
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
A novel approach to the study of hepatic glycogen kinetics and fractional gluconeogenesis in vivo is described. Ten healthy female subjects were fed an iso-caloric diet containing 55% carbohydrate energy with a 13C abundance of 1.083 atom percent for a 3-day baseline period; then, a diet of similar composition, but providing carbohydrate with a 13C abundance of 1.093 atom percent was started and continued for 5 days. Resting respiratory gas exchanges, urinary nitrogen excretion, breath 13CO2 and plasma 13C glucose were measured every morning in the fasting state. The enrichment in 13C of hepatic glycogen was calculated from these measured data. 13C glycogen enrichment increased after switching to a 13C enriched carbohydrate diet, and was identical to the 13C enrichment of dietary carbohydrates after 3 days. The time required to renew 50% of hepatic glycogen, as determined from the kinetics of 13C glycogen enrichment, was 18.9 +/- 3.6 h. Fractional gluconeogenesis, as determined from the difference between the enrichments of glucose oxidized originating from hepatic glycogen and plasma glucose 13C was 50.8 +/- 5.3%. This non-invasive method will allow the study of hepatic glycogen metabolism in insulin-resistant patients.
Keywords
Adult, Carbon Dioxide/analysis, Carbon Isotopes, Dietary Carbohydrates, Female, Gluconeogenesis, Humans, Insulin Resistance, Isotope Labeling/methods, Kinetics, Liver/metabolism, Liver Glycogen/metabolism, Reference Values
Pubmed
Web of science
Open Access
Yes
Create date
24/01/2008 13:36
Last modification date
20/08/2019 14:09
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