Identification of clonally expanded T cells in rheumatoid arthritis using a sequence enrichment nuclease assay.

Détails

ID Serval
serval:BIB_5471
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Identification of clonally expanded T cells in rheumatoid arthritis using a sequence enrichment nuclease assay.
Périodique
Journal of Clinical Investigation
Auteur(s)
González-Quintial R., Baccalá R., Pope R.M., Theofilopoulos A.N.
ISSN
0021-9738 (Print)
ISSN-L
0021-9738
Statut éditorial
Publié
Date de publication
1996
Volume
97
Numéro
5
Pages
1335-1343
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Résumé
Identification of expanded clones engaged in immune and autoimmune responses is still imperfect, since they are often diluted by irrelevant cells expressing diverse specificities. To efficiently characterize T cell receptors expressed by clonally expanded lymphocytes in rheumatoid arthritis (RA) and other inflammatory conditions, we developed an assay system, termed sequence enrichment nuclease assay (SENA). Key elements of SENA are the efficiency of heat-denatured DNA strand reassociation, which increases exponentially with concentration, and the elimination of unhybridized sequences by single-strand-specific DNase. T cell clonal expansions were identified primarily in synovial fluids, but also in peripheral blood of RA patients. Synovial fluids had more prominent expansions in the CD8 than the CD4 subset, whereas clonal expansions in the CD4 subset predominated among peripheral blood lymphocytes. Dominant clones exhibited diverse sequences with no clear conservation of junctional motifs, although the same amino acid sequence was identified in two patients. In most instances, dominant clones in the blood were discordant to those in the corresponding synovial fluid, suggesting local stimulation or preferential sequestration of T cells displaying particular specifities.
Mots-clé
Arthritis, Rheumatoid/immunology, Base Sequence, Deoxyribonucleases/pharmacology, Humans, Molecular Sequence Data, Receptors, Antigen, T-Cell, alpha-beta/genetics, Sensitivity and Specificity, T-Lymphocytes/immunology
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 13:42
Dernière modification de la notice
20/08/2019 15:09
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