Identification of clonally expanded T cells in rheumatoid arthritis using a sequence enrichment nuclease assay.

Details

Serval ID
serval:BIB_5471
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Identification of clonally expanded T cells in rheumatoid arthritis using a sequence enrichment nuclease assay.
Journal
Journal of Clinical Investigation
Author(s)
González-Quintial R., Baccalá R., Pope R.M., Theofilopoulos A.N.
ISSN
0021-9738 (Print)
ISSN-L
0021-9738
Publication state
Published
Issued date
1996
Volume
97
Number
5
Pages
1335-1343
Language
english
Notes
Publication types: Journal Article ; Research Support, U.S. Gov't, Non-P.H.S. ; Research Support, U.S. Gov't, P.H.S.
Publication Status: ppublish
Abstract
Identification of expanded clones engaged in immune and autoimmune responses is still imperfect, since they are often diluted by irrelevant cells expressing diverse specificities. To efficiently characterize T cell receptors expressed by clonally expanded lymphocytes in rheumatoid arthritis (RA) and other inflammatory conditions, we developed an assay system, termed sequence enrichment nuclease assay (SENA). Key elements of SENA are the efficiency of heat-denatured DNA strand reassociation, which increases exponentially with concentration, and the elimination of unhybridized sequences by single-strand-specific DNase. T cell clonal expansions were identified primarily in synovial fluids, but also in peripheral blood of RA patients. Synovial fluids had more prominent expansions in the CD8 than the CD4 subset, whereas clonal expansions in the CD4 subset predominated among peripheral blood lymphocytes. Dominant clones exhibited diverse sequences with no clear conservation of junctional motifs, although the same amino acid sequence was identified in two patients. In most instances, dominant clones in the blood were discordant to those in the corresponding synovial fluid, suggesting local stimulation or preferential sequestration of T cells displaying particular specifities.
Keywords
Arthritis, Rheumatoid/immunology, Base Sequence, Deoxyribonucleases/pharmacology, Humans, Molecular Sequence Data, Receptors, Antigen, T-Cell, alpha-beta/genetics, Sensitivity and Specificity, T-Lymphocytes/immunology
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 12:42
Last modification date
20/08/2019 14:09
Usage data