Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue.

Détails

ID Serval
serval:BIB_542C6908C589
Type
Partie de livre
Sous-type
Chapitre: chapitre ou section
Collection
Publications
Institution
Titre
Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue.
Titre du livre
Methods in molecular biology
Auteur⸱e⸱s
Berezowska S., Galván J.A.
Editeur
Springer
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2023
Peer-reviewed
Oui
Volume
2566
Pages
133-139
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Autophagy is a highly conserved cellular mechanism of "self-digestion," ensuring cellular homeostasis and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy. LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions.
Mots-clé
Autophagy, Immunohistochemistry, LC3, Sequestosome 1, p62/SQSTM1
Pubmed
Création de la notice
26/09/2022 7:35
Dernière modification de la notice
29/11/2022 6:49
Données d'usage