Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue.

Details

Serval ID
serval:BIB_542C6908C589
Type
A part of a book
Publication sub-type
Chapter: chapter ou part
Collection
Publications
Institution
Title
Immunohistochemical Detection of the Autophagy Markers LC3 and p62/SQSTM1 in Formalin-Fixed and Paraffin-Embedded Tissue.
Title of the book
Methods in molecular biology
Author(s)
Berezowska S., Galván J.A.
Publisher
Springer
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Publication state
Published
Issued date
2023
Peer-reviewed
Oui
Volume
2566
Pages
133-139
Language
english
Notes
Publication types: Journal Article
Publication Status: ppublish
Abstract
Autophagy is a highly conserved cellular mechanism of "self-digestion," ensuring cellular homeostasis and playing a role in many diseases including cancer. As a stress response mechanism, it may also be involved in cellular response to therapy. LC3 and Sequestosome 1 (p62/SQSTM1) are among the most widely used markers to monitor autophagy and can be visualized in formalin-fixed and paraffin-embedded tissue by immunohistochemistry. Here we describe a validated staining protocol using an automated staining system available in many routine pathology laboratories, enabling high-throughput staining under standardized conditions.
Keywords
Autophagy, Immunohistochemistry, LC3, Sequestosome 1, p62/SQSTM1
Pubmed
Create date
26/09/2022 7:35
Last modification date
29/11/2022 6:49
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