Traceable Impedance-Based Dispensing and Cloning of Living Single Cells.

Détails

ID Serval
serval:BIB_5343783783FB
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Traceable Impedance-Based Dispensing and Cloning of Living Single Cells.
Périodique
SLAS technology
Auteur⸱e⸱s
Muller G., Bonzon D., Claudinot S., Rochat A., Renaud P., Barrandon Y.
ISSN
2472-6311 (Electronic)
ISSN-L
2472-6303
Statut éditorial
Publié
Date de publication
06/2020
Peer-reviewed
Oui
Volume
25
Numéro
3
Pages
215-221
Langue
anglais
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Résumé
Single-cell cloning is essential in stem cell biology, cancer research, and biotechnology. Regulatory agencies now require an indisputable proof of clonality that current technologies do not readily provide. Here, we report a one-step cloning method using an engineered pipet combined with an impedance-based sensing tip. This technology permits the efficient and traceable isolation of living cells, stem cells, and cancer stem cells that can be individually expanded in culture and transplanted.
Mots-clé
3T3 Cells, Animals, CHO Cells, Cell Line, Tumor, Cell Survival, Cloning, Molecular, Cricetulus, Electric Impedance, Humans, Mice, Rats, Single-Cell Analysis, Stem Cells/cytology, automated biology, engineering, microfluidics, microtechnology, systems
Pubmed
Web of science
Création de la notice
20/02/2020 16:29
Dernière modification de la notice
06/08/2021 6:37
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