Traceable Impedance-Based Dispensing and Cloning of Living Single Cells.

Details

Serval ID
serval:BIB_5343783783FB
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Traceable Impedance-Based Dispensing and Cloning of Living Single Cells.
Journal
SLAS technology
Author(s)
Muller G., Bonzon D., Claudinot S., Rochat A., Renaud P., Barrandon Y.
ISSN
2472-6311 (Electronic)
ISSN-L
2472-6303
Publication state
Published
Issued date
06/2020
Peer-reviewed
Oui
Volume
25
Number
3
Pages
215-221
Language
english
Notes
Publication types: Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
Single-cell cloning is essential in stem cell biology, cancer research, and biotechnology. Regulatory agencies now require an indisputable proof of clonality that current technologies do not readily provide. Here, we report a one-step cloning method using an engineered pipet combined with an impedance-based sensing tip. This technology permits the efficient and traceable isolation of living cells, stem cells, and cancer stem cells that can be individually expanded in culture and transplanted.
Keywords
3T3 Cells, Animals, CHO Cells, Cell Line, Tumor, Cell Survival, Cloning, Molecular, Cricetulus, Electric Impedance, Humans, Mice, Rats, Single-Cell Analysis, Stem Cells/cytology, automated biology, engineering, microfluidics, microtechnology, systems
Pubmed
Web of science
Create date
20/02/2020 15:29
Last modification date
06/08/2021 5:37
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