Quantitative Cryo-Electron Tomography.
Détails
ID Serval
serval:BIB_52BC437951B4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Quantitative Cryo-Electron Tomography.
Périodique
Frontiers in molecular biosciences
ISSN
2296-889X (Print)
ISSN-L
2296-889X
Statut éditorial
Publié
Date de publication
2022
Peer-reviewed
Oui
Volume
9
Pages
934465
Langue
anglais
Notes
Publication types: Journal Article ; Review
Publication Status: epublish
Publication Status: epublish
Résumé
The three-dimensional organization of biomolecules important for the functioning of all living systems can be determined by cryo-electron tomography imaging under native biological contexts. Cryo-electron tomography is continually expanding and evolving, and the development of new methods that use the latest technology for sample thinning is enabling the visualization of ever larger and more complex biological systems, allowing imaging across scales. Quantitative cryo-electron tomography possesses the capability of visualizing the impact of molecular and environmental perturbations in subcellular structure and function to understand fundamental biological processes. This review provides an overview of current hardware and software developments that allow quantitative cryo-electron tomography studies and their limitations and how overcoming them may allow us to unleash the full power of cryo-electron tomography.
Mots-clé
cell biology, cryo-electron tomography, image processing, in situ structural biology, quantitative cryo-electron tomography
Pubmed
Web of science
Open Access
Oui
Création de la notice
22/07/2024 11:29
Dernière modification de la notice
23/07/2024 6:57