Quantitative Cryo-Electron Tomography.
Details
Serval ID
serval:BIB_52BC437951B4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Quantitative Cryo-Electron Tomography.
Journal
Frontiers in molecular biosciences
ISSN
2296-889X (Print)
ISSN-L
2296-889X
Publication state
Published
Issued date
2022
Peer-reviewed
Oui
Volume
9
Pages
934465
Language
english
Notes
Publication types: Journal Article ; Review
Publication Status: epublish
Publication Status: epublish
Abstract
The three-dimensional organization of biomolecules important for the functioning of all living systems can be determined by cryo-electron tomography imaging under native biological contexts. Cryo-electron tomography is continually expanding and evolving, and the development of new methods that use the latest technology for sample thinning is enabling the visualization of ever larger and more complex biological systems, allowing imaging across scales. Quantitative cryo-electron tomography possesses the capability of visualizing the impact of molecular and environmental perturbations in subcellular structure and function to understand fundamental biological processes. This review provides an overview of current hardware and software developments that allow quantitative cryo-electron tomography studies and their limitations and how overcoming them may allow us to unleash the full power of cryo-electron tomography.
Keywords
cell biology, cryo-electron tomography, image processing, in situ structural biology, quantitative cryo-electron tomography
Pubmed
Web of science
Open Access
Yes
Create date
22/07/2024 11:29
Last modification date
23/07/2024 6:57