Un nouveau modèle in vitro permet d'employer le même hypothalamus pour des investigations morphologiques et functionnelles [A new in vitro model allows use of the same hypothalamus for morphological and functional studies].
Détails
ID Serval
serval:BIB_526688E2388C
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Un nouveau modèle in vitro permet d'employer le même hypothalamus pour des investigations morphologiques et functionnelles [A new in vitro model allows use of the same hypothalamus for morphological and functional studies].
Périodique
Annales d'endocrinologie
ISSN
0003-4266 (Print)
ISSN-L
0003-4266
Statut éditorial
Publié
Date de publication
1987
Peer-reviewed
Oui
Volume
48
Numéro
5
Pages
438-440
Langue
français
Notes
Publication types: English Abstract ; In Vitro ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Publication Status: ppublish
Résumé
A model for combined morphological and functional investigations on the isolated rat mediobasal hypothalamus has been developed. Under these conditions of incubation, the hypothalamic tissue is well preserved, on the basis of photonic and electronic microscopic examinations. This model has been used to study the LHRH system in the rat. LHRH release has been measured in the incubation medium under basal conditions and after KCl-induced depolarisation; the, LHRH has been localized by immunohistochemistry on the hypothalamic fragment.
Mots-clé
Animals, Electrophysiology, Gonadotropin-Releasing Hormone/analysis, Gonadotropin-Releasing Hormone/secretion, Hypothalamus, Middle/anatomy & histology, Hypothalamus, Middle/drug effects, Immunohistochemistry, Male, Potassium Chloride/pharmacology, Rats, Rats, Inbred Strains, Somatostatin/analysis, Thyrotropin-Releasing Hormone/analysis
Pubmed
Web of science
Création de la notice
11/02/2008 15:11
Dernière modification de la notice
20/08/2019 15:07
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