Un nouveau modèle in vitro permet d'employer le même hypothalamus pour des investigations morphologiques et functionnelles [A new in vitro model allows use of the same hypothalamus for morphological and functional studies].

Details

Serval ID
serval:BIB_526688E2388C
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Un nouveau modèle in vitro permet d'employer le même hypothalamus pour des investigations morphologiques et functionnelles [A new in vitro model allows use of the same hypothalamus for morphological and functional studies].
Journal
Annales d'endocrinologie
Author(s)
Bestetti G.E., Boujon C.E., Reymond M.J., Rossi G.L.
ISSN
0003-4266 (Print)
ISSN-L
0003-4266
Publication state
Published
Issued date
1987
Peer-reviewed
Oui
Volume
48
Number
5
Pages
438-440
Language
french
Notes
Publication types: English Abstract ; In Vitro ; Journal Article ; Research Support, Non-U.S. Gov't
Publication Status: ppublish
Abstract
A model for combined morphological and functional investigations on the isolated rat mediobasal hypothalamus has been developed. Under these conditions of incubation, the hypothalamic tissue is well preserved, on the basis of photonic and electronic microscopic examinations. This model has been used to study the LHRH system in the rat. LHRH release has been measured in the incubation medium under basal conditions and after KCl-induced depolarisation; the, LHRH has been localized by immunohistochemistry on the hypothalamic fragment.
Keywords
Animals, Electrophysiology, Gonadotropin-Releasing Hormone/analysis, Gonadotropin-Releasing Hormone/secretion, Hypothalamus, Middle/anatomy & histology, Hypothalamus, Middle/drug effects, Immunohistochemistry, Male, Potassium Chloride/pharmacology, Rats, Rats, Inbred Strains, Somatostatin/analysis, Thyrotropin-Releasing Hormone/analysis
Pubmed
Web of science
Create date
11/02/2008 14:11
Last modification date
20/08/2019 14:07
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