A role for the POU-III transcription factor Brn-4 in the regulation of striatal neuron precursor differentiation
Détails
ID Serval
serval:BIB_4FC2091A8884
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
A role for the POU-III transcription factor Brn-4 in the regulation of striatal neuron precursor differentiation
Périodique
EMBO Journal
ISSN
0261-4189 (Print)
Statut éditorial
Publié
Date de publication
01/1999
Volume
18
Numéro
2
Pages
444-56
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jan 15
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jan 15
Résumé
Both insulin-like growth factor-I (IGF-I) and brain-derived neurotrophic factor (BDNF) induce the differentiation of post-mitotic neuronal precursors, derived from embryonic day 14 (E14) mouse striatal multipotent stem cells. Here we ask whether this differentiation is mediated by a member of the POU-III class of neural transcription factors. Exposure of stem cell progeny to either IGF-I or BDNF resulted in a rapid upregulation of Brn-4 mRNA and protein. Indirect immunocytochemistry with Brn-4 antiserum showed that the protein was expressed in newly generated neurons. Other POU-III genes, such as Brn-1 and Brn-2, did not exhibit this upregulation. Basic FGF, a mitogen for these neuronal precursors, did not stimulate Brn-4 expression. In the E14 mouse striatum, Brn-4-immunoreactive cells formed a boundary between the nestin-immunoreactive cells of the ventricular zone and the beta-tubulin-immunoreactive neurons migrating into the mantle zone. Loss of Brn-4 function during the differentiation of stem cell-derived or primary E14 striatal neuron precursors, by inclusion of antisense oligonucleotides, caused a reduction in the number of beta-tubulin-immunoreactive neurons. These findings suggest that Brn-4 mediates, at least in part, the actions of epigenetic signals that induce striatal neuron-precursor differentiation.
Mots-clé
Animals
Base Sequence
Brain-Derived Neurotrophic Factor/pharmacology
Cell Differentiation/drug effects
Corpus Striatum/*cytology/*metabolism
DNA Primers/genetics
DNA, Antisense/genetics/pharmacology
*DNA-Binding Proteins
Insulin-Like Growth Factor I/pharmacology
Mice
*Nerve Tissue Proteins
Neurons/*cytology/drug effects/*metabolism
POU Domain Factors
Stem Cells/*cytology/drug effects/*metabolism
Transcription Factors/genetics/*metabolism
Pubmed
Web of science
Open Access
Oui
Création de la notice
28/01/2008 13:31
Dernière modification de la notice
20/08/2019 15:05