A role for the POU-III transcription factor Brn-4 in the regulation of striatal neuron precursor differentiation

Details

Serval ID
serval:BIB_4FC2091A8884
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
A role for the POU-III transcription factor Brn-4 in the regulation of striatal neuron precursor differentiation
Journal
EMBO Journal
Author(s)
Shimazaki  T., Arsenijevic  Y., Ryan  A. K., Rosenfeld  M. G., Weiss  S.
ISSN
0261-4189 (Print)
Publication state
Published
Issued date
01/1999
Volume
18
Number
2
Pages
444-56
Notes
In Vitro
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S. --- Old month value: Jan 15
Abstract
Both insulin-like growth factor-I (IGF-I) and brain-derived neurotrophic factor (BDNF) induce the differentiation of post-mitotic neuronal precursors, derived from embryonic day 14 (E14) mouse striatal multipotent stem cells. Here we ask whether this differentiation is mediated by a member of the POU-III class of neural transcription factors. Exposure of stem cell progeny to either IGF-I or BDNF resulted in a rapid upregulation of Brn-4 mRNA and protein. Indirect immunocytochemistry with Brn-4 antiserum showed that the protein was expressed in newly generated neurons. Other POU-III genes, such as Brn-1 and Brn-2, did not exhibit this upregulation. Basic FGF, a mitogen for these neuronal precursors, did not stimulate Brn-4 expression. In the E14 mouse striatum, Brn-4-immunoreactive cells formed a boundary between the nestin-immunoreactive cells of the ventricular zone and the beta-tubulin-immunoreactive neurons migrating into the mantle zone. Loss of Brn-4 function during the differentiation of stem cell-derived or primary E14 striatal neuron precursors, by inclusion of antisense oligonucleotides, caused a reduction in the number of beta-tubulin-immunoreactive neurons. These findings suggest that Brn-4 mediates, at least in part, the actions of epigenetic signals that induce striatal neuron-precursor differentiation.
Keywords
Animals Base Sequence Brain-Derived Neurotrophic Factor/pharmacology Cell Differentiation/drug effects Corpus Striatum/*cytology/*metabolism DNA Primers/genetics DNA, Antisense/genetics/pharmacology *DNA-Binding Proteins Insulin-Like Growth Factor I/pharmacology Mice *Nerve Tissue Proteins Neurons/*cytology/drug effects/*metabolism POU Domain Factors Stem Cells/*cytology/drug effects/*metabolism Transcription Factors/genetics/*metabolism
Pubmed
Web of science
Open Access
Yes
Create date
28/01/2008 12:31
Last modification date
20/08/2019 14:05
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