Extracellular protease and phospholipase C are controlled by the global regulatory gene gacA in the biocontrol strain Pseudomonas fluorescens CHA0.

Détails

ID Serval
serval:BIB_4E321746E7E8
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Extracellular protease and phospholipase C are controlled by the global regulatory gene gacA in the biocontrol strain Pseudomonas fluorescens CHA0.
Périodique
FEMS Microbiology Letters
Auteur⸱e⸱s
Sacherer P., Défago G., Haas D.
ISSN
0378-1097 (Print)
ISSN-L
0378-1097
Statut éditorial
Publié
Date de publication
1994
Volume
116
Numéro
2
Pages
155-160
Langue
anglais
Résumé
Pseudomonas fluorescens strain CHA0 protects plants from various root diseases. Antibiotic metabolites synthesized by this strain play an important role in disease suppression; their production is mediated by the global activator gene gacA. Here we show by complementation that the gacA gene is also essential for the expression of two extracellular enzymes in P. fluorescens CHA0: phospholipase C and a 47-kDa metalloprotease. In contrast, the production of another exoenzyme, lipase, is not regulated by the gacA gene. Protease, phospholipase and antibiotics of P. fluorescens are all known to be optimally produced at the end of exponential growth; thus, the gacA gene appears to be a general stationary-phase regulator.
Mots-clé
Endopeptidases/chemistry, Endopeptidases/genetics, Gene Expression Regulation, Bacterial/genetics, Genes, Bacterial/genetics, Genes, Regulator/genetics, Genetic Complementation Test, Molecular Weight, Mutation, Pseudomonas fluorescens/enzymology, Pseudomonas fluorescens/genetics, Type C Phospholipases/genetics, Type C Phospholipases/metabolism
Pubmed
Web of science
Création de la notice
25/01/2008 18:01
Dernière modification de la notice
20/08/2019 15:03
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