In vitro decrease of glomerular heparan sulfate by lymphocytes from idiopathic nephrotic syndrome patients
Détails
ID Serval
serval:BIB_4DF8C86BED16
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
In vitro decrease of glomerular heparan sulfate by lymphocytes from idiopathic nephrotic syndrome patients
Périodique
Kidney International
ISSN
0085-2538
Statut éditorial
Publié
Date de publication
03/2001
Peer-reviewed
Oui
Volume
59
Numéro
3
Pages
913-22
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Research Support, Non-U.S. Gov't --- Old month value: Mar
Résumé
BACKGROUND: Lymphocytes are involved in the physiopathologic mechanism of idiopathic nephrotic syndrome (INS). We have recently demonstrated that plasma from patients with INS decreases human glomerular epithelial cell (GEC) glycosaminoglycans (GAGs), particularly heparan sulfates (HS) in vitro. In this study we investigate the effect of peripheral blood lymphocytes (PBL) from INS patients on glomerular cell GAG and HS. METHODS: Human GECs were cultured with total peripheral blood mononuclear cells (PBMCs), PBL, and monocytes from patients and controls. The amounts of GAG and HS were assessed using a cationic membrane after metabolic labeling. RESULTS: In coculture with GECs, mononuclear cells from controls decreased total epithelial cell GAG (-30% with PBMC, P < 0.05; -25% with PBL, P < 0.02; -19% with monocytes, P < 0.05). Particularly HSs were decreased (-36% with PBMC, P < 0.05; -27% with PBL, P < 0.02; and -19% with monocytes, P < 0.05). When GECs were in coculture with PBL from INS patients, the decrease in GAG and HS was significantly greater in comparison to control PBL (-10%, P < 0.02; -10%, P < 0.02, respectively, for GAG and HS). Moreover, supernatants of stimulated PBMCs from patients decreased also GAG and HS in comparison with controls (-13%, P < 0.02; -15%, P < 0.02, respectively, for GAG and HS). CONCLUSION: These data provide direct evidence that PBLs from INS patients are able to decrease GEC HS as previously shown with plasma from patients. This might be instrumental in the onset of albuminuria.
Mots-clé
Cells, Cultured
Child
Chondroitin Sulfates/metabolism
Glycosaminoglycans/chemistry/metabolism
Heparitin Sulfate/*antagonists & inhibitors
Humans
Kidney Glomerulus/*metabolism/pathology
Lymphocytes/*physiology
Monocytes/physiology
Nephrotic Syndrome/*metabolism/pathology
Polymers/metabolism
Reference Values
Pubmed
Web of science
Open Access
Oui
Création de la notice
15/01/2008 15:28
Dernière modification de la notice
20/08/2019 15:03