In vitro decrease of glomerular heparan sulfate by lymphocytes from idiopathic nephrotic syndrome patients

Details

Serval ID
serval:BIB_4DF8C86BED16
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
In vitro decrease of glomerular heparan sulfate by lymphocytes from idiopathic nephrotic syndrome patients
Journal
Kidney International
Author(s)
Birmele  B., Thibault  G., Nivet  H., de Agostini  A., Girardin  E. P.
ISSN
0085-2538
Publication state
Published
Issued date
03/2001
Peer-reviewed
Oui
Volume
59
Number
3
Pages
913-22
Notes
Journal Article
Research Support, Non-U.S. Gov't --- Old month value: Mar
Abstract
BACKGROUND: Lymphocytes are involved in the physiopathologic mechanism of idiopathic nephrotic syndrome (INS). We have recently demonstrated that plasma from patients with INS decreases human glomerular epithelial cell (GEC) glycosaminoglycans (GAGs), particularly heparan sulfates (HS) in vitro. In this study we investigate the effect of peripheral blood lymphocytes (PBL) from INS patients on glomerular cell GAG and HS. METHODS: Human GECs were cultured with total peripheral blood mononuclear cells (PBMCs), PBL, and monocytes from patients and controls. The amounts of GAG and HS were assessed using a cationic membrane after metabolic labeling. RESULTS: In coculture with GECs, mononuclear cells from controls decreased total epithelial cell GAG (-30% with PBMC, P < 0.05; -25% with PBL, P < 0.02; -19% with monocytes, P < 0.05). Particularly HSs were decreased (-36% with PBMC, P < 0.05; -27% with PBL, P < 0.02; and -19% with monocytes, P < 0.05). When GECs were in coculture with PBL from INS patients, the decrease in GAG and HS was significantly greater in comparison to control PBL (-10%, P < 0.02; -10%, P < 0.02, respectively, for GAG and HS). Moreover, supernatants of stimulated PBMCs from patients decreased also GAG and HS in comparison with controls (-13%, P < 0.02; -15%, P < 0.02, respectively, for GAG and HS). CONCLUSION: These data provide direct evidence that PBLs from INS patients are able to decrease GEC HS as previously shown with plasma from patients. This might be instrumental in the onset of albuminuria.
Keywords
Cells, Cultured Child Chondroitin Sulfates/metabolism Glycosaminoglycans/chemistry/metabolism Heparitin Sulfate/*antagonists & inhibitors Humans Kidney Glomerulus/*metabolism/pathology Lymphocytes/*physiology Monocytes/physiology Nephrotic Syndrome/*metabolism/pathology Polymers/metabolism Reference Values
Pubmed
Web of science
Open Access
Yes
Create date
15/01/2008 14:28
Last modification date
20/08/2019 14:03
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