Modulation of the plasminogen activation system by inflammatory cytokines in human colon carcinoma cells.
Détails
Etat: Public
Version: Final published version
Licence: Non spécifiée
ID Serval
serval:BIB_4518
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Modulation of the plasminogen activation system by inflammatory cytokines in human colon carcinoma cells.
Périodique
British Journal of Cancer
ISSN
0007-0920
Statut éditorial
Publié
Date de publication
1996
Volume
74
Numéro
6
Pages
846-852
Langue
anglais
Résumé
Inflammation may promote malignant invasion by enhancing cancer cell-associated proteolysis. Here we present the effect of inflammatory cytokines on the plasminogen activation system of eight human colon carcinoma cell lines. Tumour necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) increased in several, but not all, cell lines the production of urokinase-type plasminogen activator (uPA), tissue-type PA (tPA) and plasminogen activator inhibitor type 1 (PAI-1) as analysed by zymography, enzyme immunoassays and Northern analysis. Interleukin 6 (IL-6) had no effect. uPA receptor (uPAR) mRNA levels were also upregulated. However, each individual cell line responded differently following exposure to TNF-alpha or IL-1 beta. For example, there was a dose-dependent up-regulation of uPA and PAI-1 in SW 620 cells, whereas increased uPA production in SW 1116 cells was not accompanied by an increase in PAI-1. The TNF-alpha stimulatory effect was blocked by anti-TNF-alpha Fab fragments. All cell lines expressed both types of TNF receptor mRNAs, whereas no transcript for TNF-alpha, IL-1 beta, IL-6, IL-6 receptor or the IL-1 receptors was found. Our results demonstrate that TNF-alpha and IL-1 beta stimulate the plasminogen activation system in tumour cell but the responses differed even in cells derived from the same tissue origin.
Mots-clé
Blotting, Northern, Colonic Neoplasms/metabolism, Cytokines/pharmacology, Dose-Response Relationship, Drug, Humans, Interleukin-1/pharmacology, Plasminogen Activator Inhibitor 1/biosynthesis, Plasminogen Activators/biosynthesis, Receptors, Tumor Necrosis Factor/analysis, Tumor Cells, Cultured, Tumor Necrosis Factor-alpha/pharmacology
OAI-PMH
Pubmed
Web of science
Open Access
Oui
Création de la notice
19/11/2007 13:40
Dernière modification de la notice
20/08/2019 14:49