Modulation of the plasminogen activation system by inflammatory cytokines in human colon carcinoma cells.
Details
State: Public
Version: Final published version
License: Not specified
Serval ID
serval:BIB_4518
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Modulation of the plasminogen activation system by inflammatory cytokines in human colon carcinoma cells.
Journal
British Journal of Cancer
ISSN
0007-0920
Publication state
Published
Issued date
1996
Volume
74
Number
6
Pages
846-852
Language
english
Abstract
Inflammation may promote malignant invasion by enhancing cancer cell-associated proteolysis. Here we present the effect of inflammatory cytokines on the plasminogen activation system of eight human colon carcinoma cell lines. Tumour necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) increased in several, but not all, cell lines the production of urokinase-type plasminogen activator (uPA), tissue-type PA (tPA) and plasminogen activator inhibitor type 1 (PAI-1) as analysed by zymography, enzyme immunoassays and Northern analysis. Interleukin 6 (IL-6) had no effect. uPA receptor (uPAR) mRNA levels were also upregulated. However, each individual cell line responded differently following exposure to TNF-alpha or IL-1 beta. For example, there was a dose-dependent up-regulation of uPA and PAI-1 in SW 620 cells, whereas increased uPA production in SW 1116 cells was not accompanied by an increase in PAI-1. The TNF-alpha stimulatory effect was blocked by anti-TNF-alpha Fab fragments. All cell lines expressed both types of TNF receptor mRNAs, whereas no transcript for TNF-alpha, IL-1 beta, IL-6, IL-6 receptor or the IL-1 receptors was found. Our results demonstrate that TNF-alpha and IL-1 beta stimulate the plasminogen activation system in tumour cell but the responses differed even in cells derived from the same tissue origin.
Keywords
Blotting, Northern, Colonic Neoplasms/metabolism, Cytokines/pharmacology, Dose-Response Relationship, Drug, Humans, Interleukin-1/pharmacology, Plasminogen Activator Inhibitor 1/biosynthesis, Plasminogen Activators/biosynthesis, Receptors, Tumor Necrosis Factor/analysis, Tumor Cells, Cultured, Tumor Necrosis Factor-alpha/pharmacology
OAI-PMH
Pubmed
Web of science
Open Access
Yes
Create date
19/11/2007 13:40
Last modification date
20/08/2019 14:49