Unraveling cartilage degeneration through synergistic effects of hydrostatic pressure and biomimetic temperature increase.

Détails

ID Serval
serval:BIB_3D5ADEF0B7F4
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Titre
Unraveling cartilage degeneration through synergistic effects of hydrostatic pressure and biomimetic temperature increase.
Périodique
iScience
Auteur⸱e⸱s
Guo Y., Stampoultzis T., Nasrollahzadeh N., Karami P., Rana V.K., Applegate L., Pioletti D.P.
ISSN
2589-0042 (Electronic)
ISSN-L
2589-0042
Statut éditorial
Publié
Date de publication
15/12/2023
Peer-reviewed
Oui
Volume
26
Numéro
12
Pages
108519
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: epublish
Résumé
Cartilage degeneration, typically viewed as an irreversible, vicious cycle, sees a significant reduction in two essential biophysical cues: the well-established hydrostatic pressure (HP) and the recently discovered transient temperature increase. Our study aimed to evaluate the combined influence of these cues on maintaining cartilage homeostasis. To achieve this, we developed a customized bioreactor, designed to mimic the specific hydrostatic pressure and transient thermal increase experienced during human knee physiological activities. This system enabled us to investigate the response of human 3D-cultured chondrocytes and human cartilage explants to either isolated or combined hydrostatic pressure and thermal stimuli. Our study found that chondroinduction (SOX9, aggrecan, and sulfated glycosaminoglycan) and chondroprotection (HSP70) reached maximum expression levels when hydrostatic pressure and transient thermal increase acted in tandem, underscoring the critical role of these combined cues in preserving cartilage homeostasis. These findings led us to propose a refined model of the vicious cycle of cartilage degeneration.
Mots-clé
Bioengineering, Biological sciences, Natural sciences, Physiology
Pubmed
Web of science
Open Access
Oui
Création de la notice
10/01/2024 12:19
Dernière modification de la notice
27/01/2024 8:36
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