Unraveling cartilage degeneration through synergistic effects of hydrostatic pressure and biomimetic temperature increase.

Details

Serval ID
serval:BIB_3D5ADEF0B7F4
Type
Article: article from journal or magazin.
Collection
Publications
Institution
Title
Unraveling cartilage degeneration through synergistic effects of hydrostatic pressure and biomimetic temperature increase.
Journal
iScience
Author(s)
Guo Y., Stampoultzis T., Nasrollahzadeh N., Karami P., Rana V.K., Applegate L., Pioletti D.P.
ISSN
2589-0042 (Electronic)
ISSN-L
2589-0042
Publication state
Published
Issued date
15/12/2023
Peer-reviewed
Oui
Volume
26
Number
12
Pages
108519
Language
english
Notes
Publication types: Journal Article
Publication Status: epublish
Abstract
Cartilage degeneration, typically viewed as an irreversible, vicious cycle, sees a significant reduction in two essential biophysical cues: the well-established hydrostatic pressure (HP) and the recently discovered transient temperature increase. Our study aimed to evaluate the combined influence of these cues on maintaining cartilage homeostasis. To achieve this, we developed a customized bioreactor, designed to mimic the specific hydrostatic pressure and transient thermal increase experienced during human knee physiological activities. This system enabled us to investigate the response of human 3D-cultured chondrocytes and human cartilage explants to either isolated or combined hydrostatic pressure and thermal stimuli. Our study found that chondroinduction (SOX9, aggrecan, and sulfated glycosaminoglycan) and chondroprotection (HSP70) reached maximum expression levels when hydrostatic pressure and transient thermal increase acted in tandem, underscoring the critical role of these combined cues in preserving cartilage homeostasis. These findings led us to propose a refined model of the vicious cycle of cartilage degeneration.
Keywords
Bioengineering, Biological sciences, Natural sciences, Physiology
Pubmed
Web of science
Open Access
Yes
Create date
10/01/2024 11:19
Last modification date
27/01/2024 7:36
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