Visualization of nutritive perfusion following tourniquet ischemia in arterial pattern skin flaps: effect of vasoactive medication.

Détails

ID Serval
serval:BIB_3CBDDBD9D4D0
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Titre
Visualization of nutritive perfusion following tourniquet ischemia in arterial pattern skin flaps: effect of vasoactive medication.
Périodique
Plastic and Reconstructive Surgery
Auteur⸱e⸱s
Saetzler R.K., Lehr H.A., Barker J.H., Kamler M., Galla T.J., Messmer K.
ISSN
0032-1052 (Print)
Statut éditorial
Publié
Date de publication
1994
Volume
94
Numéro
5
Pages
652-660
Langue
anglais
Résumé
We present an experimental model that makes it possible to investigate the effects of global ischemia and reperfusion on microvascular perfusion and viability of ill-proportioned (poorly designed) arterial pattern skin flaps in hairless mice. Skin flaps were created on the ears of hairless mice by dissecting two of three nutritional vessel bundles at the ear base. Under these nonischemic conditions, 19 percent of the total flap area went on to necrose (as a result of poor flap design). Global ischemia was induced to the flap tissue for 6 hours with a tourniquet clamp directly after flap incision. The extension of perfused tissue area and flap viability were assessed at the microcirculatory level by intravital video microscopy at 1, 3, 6, and 18 hours and 7 days after reperfusion in animals treated with either normal saline (control) or the vasoactive drug buflomedil hydrochloride (3 mg/kg of body weight per day, i.v., starting 4 hours prior to flap creation and continued at daily intervals until the end of the experiments). In untreated animals (n = 18), 1 hour after clamp release we observed reperfusion of 39.55 percent (38.5/44.9) of total flap area. Reperfusion remained unchanged within the following 5 hours. Within the next 12 hours, reperfused flap area was dramatically reduced to 21.9 percent (15.1/58.4). Seven days thereafter, only 18.8 percent (10.9/42.2) of total flap area remained viable. In contrast, we found in buflomedil-treated animals (n = 18) that 57.3 percent (53.5/62.9) of the total flap tissue was reperfused within the first hour after clamp release (p < 0.01).(ABSTRACT TRUNCATED AT 250 WORDS)
Mots-clé
Animals, Ear, External/blood supply, Male, Mice, Mice, Hairless, Microcirculation/drug effects, Microcirculation/physiology, Microscopy, Fluorescence, Pyrrolidines/therapeutic use, Reperfusion Injury/pathology, Reperfusion Injury/prevention & control, Surgical Flaps/methods, Surgical Flaps/pathology, Tourniquets, Vasodilator Agents/therapeutic use
Pubmed
Création de la notice
25/11/2011 19:16
Dernière modification de la notice
20/08/2019 13:32
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