Isolation and Characterization of Mouse Neutrophils.

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ID Serval
serval:BIB_3C5A7314BDB7
Type
Article: article d'un périodique ou d'un magazine.
Collection
Publications
Institution
Production externe
Titre
Isolation and Characterization of Mouse Neutrophils.
Périodique
Methods in molecular biology
Auteur⸱e⸱s
Ubags NDJ, Suratt B.T.
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
1809
Pages
45-57
Langue
anglais
Notes
Publication types: Journal Article
Publication Status: ppublish
Résumé
Isolation of murine neutrophils from several anatomical compartments allows for functional characterization and analysis of these cells. Here we describe the isolation of bone marrow, peripheral blood, and lung airspace and interstitial neutrophil populations, using density gradient separation, lavage, and flow cytometry techniques.
Mots-clé
Animals, Biomarkers, Bone Marrow Cells/cytology, Bone Marrow Cells/metabolism, Cell Separation/methods, Centrifugation, Density Gradient/methods, Flow Cytometry, Immunophenotyping/methods, Lung/cytology, Mice, Neutrophils/cytology, Neutrophils/metabolism, Blood, Density gradient, Isolation, Lung, Marrow, Neutrophils
DOI
10.1007/978-1-4939-8570-8_4
Pubmed
29987781
Création de la notice
29/04/2022 17:00
Dernière modification de la notice
30/04/2022 6:37
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