Isolation and Characterization of Mouse Neutrophils.
Détails
ID Serval
serval:BIB_3C5A7314BDB7
Type
Partie de livre
Sous-type
Chapitre: chapitre ou section
Collection
Publications
Institution
Titre
Isolation and Characterization of Mouse Neutrophils.
Titre du livre
Methods in molecular biology
Editeur
Springer
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Statut éditorial
Publié
Date de publication
2018
Peer-reviewed
Oui
Volume
1809
Numéro de chapitre
4
Pages
45-57
Langue
anglais
Résumé
Isolation of murine neutrophils from several anatomical compartments allows for functional characterization and analysis of these cells. Here we describe the isolation of bone marrow, peripheral blood, and lung airspace and interstitial neutrophil populations, using density gradient separation, lavage, and flow cytometry techniques.
Mots-clé
Animals, Biomarkers, Bone Marrow Cells/cytology, Bone Marrow Cells/metabolism, Cell Separation/methods, Centrifugation, Density Gradient/methods, Flow Cytometry, Immunophenotyping/methods, Lung/cytology, Mice, Neutrophils/cytology, Neutrophils/metabolism, Blood, Density gradient, Isolation, Lung, Marrow, Neutrophils
Pubmed
Création de la notice
29/04/2022 17:00
Dernière modification de la notice
29/07/2024 9:56
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