Isolation and Characterization of Mouse Neutrophils.
Details
Serval ID
serval:BIB_3C5A7314BDB7
Type
A part of a book
Publication sub-type
Chapter: chapter ou part
Collection
Publications
Institution
Title
Isolation and Characterization of Mouse Neutrophils.
Title of the book
Methods in molecular biology
Publisher
Springer
ISSN
1940-6029 (Electronic)
ISSN-L
1064-3745
Publication state
Published
Issued date
2018
Peer-reviewed
Oui
Volume
1809
Chapter
4
Pages
45-57
Language
english
Abstract
Isolation of murine neutrophils from several anatomical compartments allows for functional characterization and analysis of these cells. Here we describe the isolation of bone marrow, peripheral blood, and lung airspace and interstitial neutrophil populations, using density gradient separation, lavage, and flow cytometry techniques.
Keywords
Animals, Biomarkers, Bone Marrow Cells/cytology, Bone Marrow Cells/metabolism, Cell Separation/methods, Centrifugation, Density Gradient/methods, Flow Cytometry, Immunophenotyping/methods, Lung/cytology, Mice, Neutrophils/cytology, Neutrophils/metabolism, Blood, Density gradient, Isolation, Lung, Marrow, Neutrophils
Pubmed
Create date
29/04/2022 17:00
Last modification date
29/07/2024 9:56
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